Vegeta is an obvious one - this is the guy who for more than 10 years has been stubbornly deadnaming Goku, always calling him Kakarot despite Goku's protests. Vegeta IRL would be a right misgendering machine.
Also, in the English dub at least, Vegeta implies that Future Trunks is gay for complementing Vegeta's pink shirt (which he hates), and obviously means it as an insult.
@ChristiJunior Morag does NOT appreciate men in women's spaces. You might get her to go along initially, but the instant she sees a dick in the women's changing room she's going full TERF.
@ChristiJunior Mario Mario. When launching his number 1 enemy into a naval mine he'd yell "so long, gay Bowser!" in a show of disrespect. Clearly, Mario Mario doesn't hold homos in high regard.
Two Palutena's Guidance conversations make it crystal clear that Viridi from Kid Icarus: Uprising hates trannies. In the first ones, she objects to Yoshi being a male who lays eggs (guess her stance on Pregnant Men), and concludes by saying that "there's nothing natural about this guy!", which the Goddess of Nature obviously means as an insult. In the second one, she openly says that Mewtwo needs to be destroyed for being a freak of nature created by mad scientists.
Also, she apparently hates crybabies and depressed kids.
....so yeah, even if she didn't already hate most humans, Viridi would definitely support #TTD.
I guess most Castlevania protagonists are religious to some extent, but Reinhardt Schneider from Castlevania 64 and Legacy of Darkness is easily the most devout, and will even prevent a vampire from an heroing because "God forbids suicide". Hey, you guys know what else God forbids? :0380:
Note the Castlevania 64 also has something of a detransitioning storyline going on - sure, it's about going from vampire back to human, not going from tranny back to being normal, but aren't trannies kinda like vampires anyway? I mean, without the powers and cool-factor obviously, and a lot more pedo, but still, the comparison seems warranted.
Ingrid from Fire Emblem: Three Houses is well known for being openly racist, but did you know that she's also not too fond of gay people? When Dorothea the bisexual gold digger tries to convince her tomboy friend to dress up for social occasions, she finishes her pitch by saying that pretty clothes will make Ingrid irresistible - including to Dorothea!
Ingrid does NOT appreciate this "joke", at all, and it arguably ends their whole friendship, because their Support chain never gets past B, and ends on that decidedly sour note. Now, I'm not gonna pretend that Dorothea X Ingrid Yuri action wouldn't be hot af, but I also have to appreciate Ingrid being a good follower of Sothis and putting the thot in her place.
Monokuma from Danganronpa 1 (and by extension the Mastermind behind the bear) clearly recognizes that dressing up and pretending to be a girl doesn't actually make you one.
Tenko Chabashira from Danganronpa v3 is not only a raging misandrist, she's also implied to be a lesbian (at least she's consistent), and the girl she lusts after totally looks like a Loli (that's another kettle of fish, let's not get into that now). So what is she doing on THIS list?
Well, if you can't recognize an obvious TERF when you see one, I don't know what to tell you. Tenko IRL would be the second coming of Emma Faber (there's no doubt she'd be on Spinster), and she'd be absolutely OUTRAGED over the idea of "degenerate males" (a catchphrase of hers) pretending to be women and invading women's spaces. She'd throw trannies face-first into the mirror if she caught any of those faggots in the women's bathroom.
13 Sentinels's Tomi Kisaragi is originally from the year 2024, where the anti-tranny backlash is seemingly well underway, because she's VERY comfortable openly mocking the character Okino for his habit of dressing up like a girl, no worries whatsoever about Cancellation. She never apologizes or takes it back either.